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1. #Analyze sequencing results serial cloner pro
2. #Analyze sequencing results serial cloner verification

#Analyze sequencing results serial cloner pro

Try it! – MultiSite Gateway Pro cloning.

#Analyze sequencing results serial cloner verification

Interpret results from clone verification.Step 4: Digest/linearize a circular vector.Step 3: Specify the vector and entry clone.Option F – Use restriction enzyme cut sites to create the insert.Option E – Use the PCR product of the current primer pair as the insert.Option D – Use part of an open SeqBuilder Pro sequence file as the insert.Option C – Use an entire open SeqBuilder Pro sequence as the insert.Option B – Copy inserts from another SeqBuilder Pro cloning project.Option A – Use a sequence file on your computer as the insert.Step 1: Start a cloning project and specify the insert.Create a custom vector for directional TOPO cloning.Create a custom vector for Gateway cloning.Preliminary Step (optional): Add custom vectors to the cloning vector catalog.

Try it! – Create a style set for CDS features.Specify the appearance of features in the Feature Library Manager and Annotation Results window.Specify the features to use in auto-annotation.Create a custom feature database for auto-annotation searches.Try it! – Create and modify primers for TA cloning.Create a primer based on a selected region of sequence.View cut sites and recognition sequence.Example of showing potential cuts at ambiguous bases.Try it! – Apply a restriction enzyme to the sequence.Apply or remove enzymes in the Gel Simulation view.Try it! – Find restriction sites on either side of a feature.Enzymes with unusual cleavage properties (Bgh I and Taq II).Create sequences from features in another sequence.Create one sequence from multiple sequences.Create translations of multiple sequences.Create reverse complements of multiple sequences.Create Sequences Based on Other Sequences (Batch Editing).Open a BLAST database file using a similarity search.Specify a download folder for online search results.Open results in a Lasergene application.Open an Entrez database file using a text search.Try it! – Open an Entrez sequence by accession number.Open an Entrez database file by locus name or accession number.Specify URLS for BLAST and Entrez Searches.Open a sequence from an online database.Open a sequence from the Project window.Change the default application for opening Lasergene files.